Effect of heat stress amelioration during dry period on biological responses, proinflammatory cytokines and milk production in subsequent lactation of Surti buffaloes

Present study was conducted on 12 dry Surti buffaloes to assess the effect of heat stress amelioration during period their biological responses and milk production in subsequent lactation. Buffaloes were divided into control (n=6) treatment groups. Treatment housed only shed having wall-mounted fans, roof whitewashed with microfine lime powder open paddock covered 75% green net. At -8, -3, +1 +3 weeks calving, blood collection recording observations (physiological, skin thermography BCS) done. Milk composition daily yield recorded at every 2 up 11 lactation, respectively. Shed modification successful lowereing air temperature THI indicating thermal comfort for as also revealed by lower temperatures rectum, tympanic membrane, surface like forehead, eye, udder, coronet respiration rate. group maintained significantly higher BCS, glucose, total protein, GSH, TAS, vitro lymphocyte proliferation, neutrophil phagocytic activity levels urea, creatinine, NEFA, BHBA, SOD, MDA, cortisol, TNF-α, IL-1β, IL-6 HSP70. These results are indicative minimum negative energy balance, immune response. fat group. Providing helped abating effects not but lactation along improved yield.